Identification of nodulation promoter (nod-box) regions ofRhizobium galegae
نویسندگان
چکیده
منابع مشابه
Conservation of extended promoter regions of nodulation genes in Rhizobium.
A 47-base-pair (bp) conserved sequence in the 5'-flanking regions of three transcriptional units coding for nodulation functions (nodABC, nodEFG, and nodH) has been identified in Rhizobium meliloti strain 41. The conserved region contains subsequences of 7 bp, 5 bp, and 25 bp. The conserved 25-bp sequence was synthesized and used as a hybridization probe; three additional copies of the sequence...
متن کاملThe Rhizobium meliloti region located downstream of the nod box n6 is involved in the specific nodulation of Medicago lupulina.
Six nod box regulatory sequences are present in the Rhizobium meliloti genome. We have analysed the DNA region located downstream of nod box n6, and identified three open reading frames, designated nolQa, nolQb and nolS. LacZ fusions in these ORFs are not induced by classical nod gene inducers, which indicates that their expression either is not under the control of the nod box, or involves ano...
متن کاملDiversity and Phylogeny of Rhizobium galegae, and reflections on molecular evolution of rhizobium-legume symbiosis
List of original papers This thesis is based on the following articles, which in the text will be referred to by their Roman numerals. Additionally, some unpublished results are presented. fingerprinting as a tool to study the genetic diversity of Rhizobium galegae. diversity of rhizobia isolated from Astragalus adsurgens growing in different geographical regions of China. Molecular evolution o...
متن کاملIdentification and Characterization of Nodulation- Signaling Pathway 2, a Gene of Medicago truncatula Involved in Nod Factor Signaling
Bacterially derived Nod factor is critical in the establishment of the legume/rhizobia symbiosis. Understanding the mechanisms of Nod factor perception and signal transduction in the plant will greatly advance our understanding of this complex interaction. Here, we describe the identification of a new locus, nodulation-signaling pathway 2 (NSP2), of Medicago truncatula that is involved in Nod f...
متن کاملIdentification of Conserved Regulatory Elements in Mammalian Promoter Regions: A Case Study Using the PCK1 Promoter
A systematic phylogenetic footprinting approach was performed to identify conserved transcription factor binding sites (TFBSs) in mammalian promoter regions using human, mouse and rat sequence alignments. We found that the score distributions of most binding site models did not follow the Gaussian distribution required by many statistical methods. Therefore, we performed an empirical test to es...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1999
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1999.tb13735.x